Home / People / / Matthias Bureik

Matthias Bureik——Professor

Nationality:
GERMAN
Phone:
+8615522686706
Email:
matthias@tju.edu.cn
Office:
Room A506B, Building 24, Tianjin University
School:
School of Pharmaceutical Science and Technology
ResearcherID:
Group weblink
Education Experience
1994-1997 Ph. D. Medical Biochemistry Saarland University Med. School, Homburg, Germany
1997-2000 Postdoctoral Biochemistry Saarland University, Germany
1987-1994 Master of Science Chemistry Saarland University, Germany
Professional Experience
2001-2004 Senior Scientist (C1) Department of Biochemistry, Saarland University, Germany
2004-2008 Senior Scientist (C2) Department of Biochemistry, Saarland University, Germany
2008-2012 CEO PomBioTech GmbH, Germany
2012-2014 Lecturer & Senior Scientist Department of Biochemistry, Saarland University, Germany
Research Area

The research in the Bureik group spans two primary areas: 1) Human drug metabolizing enzymes and 2) investigation of human CYP4Z1 and the treatment of breast cancer. A primary goal in the first area involves systematic testing of all variants of drug metabolizing cytochrome P450 enzymes (CYPs or P450s; see left figure below) and UDP glycosyltransferases (UGTs) identified in Chinese patients. This is expected to aid doctors in choosing the correct dosage for patients. In the second area, the group has successfully identified CYP4Z1 to be a fatty acid in-chain hydroxylase. In addition, additional substrates of this enzyme that are both structurally different and far better suited to screening procedures than fatty acids have been identified. A primary aim is to search for compounds that can act as CYP4Z1-activated prodrugs (right figure) and have potential for treatment of breast cancer.

1.jpg2.jpg

Honors and Awards
2005 – German business magazine impulse: PomBioTech was named as one of the 25 most promising start-up companies in Germany. The award address was given by Wolfgang Clement, then German Federal Minister of Economics and Labour.
2011 – PomBioTech was selected as a finalist for Red Herring's Top 100 Europe award.
Patents
Bureik, M., J.M. Naumann & C.-A. Dragan, Method for the production of Sweet Proteins. 2010, WO/2011/124463.
Bureik M., C.-A. Dragan & A. Dragan, Method of generating HCV-derived virus-like particles. 2009, WO/2011/042551.
Bureik M., T. Hakki & R. Bernhardt, Verwendung von Inhibitoren der Aldosteron-Synthase zur Behandlung von Hyperaldosteronismus und Herz-Kreislauf-Erkrankungen. 2009, DE102009044375.
Zöllner, A. & M. Bureik, Methods for identifying potential CYP4 metabolites, inhibitors or prodrugs. 2008, WO/2010/040770.
Dragan, C.-A., D. Buchheit & M. Bureik, Drug metabolism. 2008, WO/2010/031875.
Hartmann, R.W., S. Ulmschneider, U. Muller-Vieira, M. Bureik & R. Bernhardt, Selektive Hemmstoffe der Aldosteronsynthase (CYP11B2) zur Behandlung der Herzinsuffizienz und Myokardfibrose. 2004, DE 102004035322.
Bernhardt, R., A. Bichet, M. Bureik & N. Lenz, Der "Bringer" - eine hocheffiziente Methode zur Erzeugung von Mutantenbibliotheken. 2002, DE 10260977.
Bureik, M. & R. Bernhardt, Produktion von Steroidhormonen durch rekombinante Spalthefen. 1998, DE 19826821.
Highlighted Publications
Neunzig I., C.-A. Dragan, M. Widjaja, A.E. Schwaninger, F.T. Peters, H.H. Maurer & M. Bureik (2011) Whole-cell Biotransformation Assay for Investigation of the Human Drug Metabolizing Enzyme CYP3A7. Biochim Biophys Acta 1814, 161-167.
Naumann J.M., G. Küttner & M. Bureik (2011) Expression and Secretion of a CB4-1 scFv-GFP Fusion Protein by Fission Yeast. Appl Biochem Biotechnol 163, 80-9.
Dragan C.-A., F.T. Peters, P. Bour, A.E. Schwaninger, S.M. Schaan, I. Neunzig, M. Widjaja, J. Zapp, T. Kraemer, H.H. Maurer & M. Bureik (2011) Convenient Gram-Scale Metabolite Synthesis by Engineered Fission Yeast Strains Expressing Functional Human P450 Systems. Appl Biochem Biotechnol 163, 965-980.
Naumann J.M., A. Zöllner, C.-A. Dragan, J. Messinger, J. Adam & M. Bureik (2011) Biotechnological production of 20α-dihydrodydrogesterone at pilot scale. Appl Biochem Biotechnol 165, 190-203.
Buchheit D., E.I. Schmitt, D. Bischoff, T. Ebner & M. Bureik (2011) S-Glucuronidation of 7-mercapto-4-methylcoumarin by human UDP glycosyltransferases in genetically engineered fission yeast cells. Biol Chem 392, 1089-95.
Parr, M.K., A. Zöllner, G. Fusshöller, G. Opfermann, N. Schlörer, M. Zorio, M. Bureik & W. Schänzer (2012) Unexpected contribution of cytochrome P450 enzymes CYP11B2 and CYP21, as well as CYP3A4 in xenobiotic androgen elimination - Insights from metandienone metabolism. Toxicol Lett. 213, 381-391.
Neunzig, I., M. Widjaja, F.T. Peters, H.H. Maurer, A. Hehn, F. Bourgaud & M. Bureik (2013) Coexpression of CPR from various origins enhances biotransformation activity of human CYPs in S. pombe. Appl Biochem Biotechnol. 170, 1751-1766.
Klein, T., S. Lange, N. Wilhelm, M. Bureik, T.H. Yang, E. Heinzle & K. Schneider (2014) Overcoming the metabolic burden of protein secretion in Schizosaccharomyces pombe - A quantitative approach using C-13-based metabolic flux analysis. Metab Eng. 21, 34-45.
Weyler C, M. Bureik & E. Heinzle (in press) Selective oxidation of UDP-glucose to UDP-glucuronic acid using permeabilized Schizosaccharomyces pombe expressing human UDP-glucose 6-dehydrogenase. Biotechnol Lett.
Neunzig, I.; Göhring, A.; Dragan, C.-A.; Zapp, J.; Peters, F. T.; Maurer, H. H.; Bureik, M. Production and NMR analysis of the human ibuprofen metabolite 3-hydroxyibuprofen. 2012, J. Biotechnol. 157, 417.
Neunzig, I.; Widjaja, M.; Dragan, C.-A.; Peters, F. T.; Maurer, H. H.; Bureik, M. Engineering of human CYP3A enzymes by combination of activating polymorphic variants. Appl. Biochem. Biotechnol. 2012, 168, 785.
Buchheit, D.; Dragan, C.-A.; Schmitt, E. I.; Bureik, M. Production of Ibuprofen Acyl Glucosides by Human UGT2B7. Drug Metab Dispos 2011, 39, 2174.
Zöllner A.; Buchheit, D.; Meyer, M. R.; Maurer, H. H.; Peters, F. T.; Bureik, M. Production of human phase 1 and 2 metabolites by whole-cell biotransformation with recombinant microbes. Bioanalysis 2010, 2, 1277. (Review)
Zöllner, A.; Dragan, C.-A.; Pistorius, D.; Müller, R.; Bode, H. B.; Peters, F. T.; Maurer, H. H.; Bureik, M. Human CYP4Z1 catalyzes the in-chain hydroxylation of lauric acid and myristic acid. Biol. Chem. 2009, 390, 313.
ResearcherID Publications
Year Title Author(s) Source Volume