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Françisco Raymo——Professor

School of Pharmaceutical Science and Technology
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Education Experience
1993-1996 Ph. D. Chemistry University of Birmingham
1996-1997 Postdoctoral Chemistry University of Birmingham
1997-1999 Postdoctoral Chemistry University of California, Los Angeles
Professional Experience
2000-2004 Assistant Professor University of Miami
2004-2009 Associate Professor University of Miami
2009-2018 Professor University of Miami
Research Area

The goal of our research program is the development of operating principles to activate the fluorescence of organic chromophores under either chemical or optical control. In particular, we design molecules capable of switching from a nonemissive to an emissive state, upon either acidification or illumination at an appropriate activation wavelength, and then produce fluorescence, after irradiation at a given excitation wavelength.  The former mechanism can transduce pH changes into fluorescence signals and permits the imaging of acidic intracellular compartments with excellent contrast.  The latter mechanism can activate fluorescence within a defined region of space at a specific interval of time, relying exclusively on the interplay of beams illuminating the sample at the activation and excitation wavelengths.  Such level of spatiotemporal control offers the opportunity to overcome diffraction and reconstruct fluorescence images with spatial resolution at the nanometer level as well as to monitor dynamic events in real time with the sequential acquisition of fluorescence images.  We are particularly interested in exploring these operating principles for either chemical or optical fluorescence activation to detect cancer cells, visualize intracellular substructures with nanoscaled resolution and track the translocation of species within living organisms.  Thus, our fundamental investigations on molecular switches can eventually lead to the realization of innovative imaging probes for a diversity of bioanalytical applications.

Honors and Awards
"Photochromic Compounds Based on Ring Opening and Closing of a [1,3]Oxazine Compound": F. M. Raymo, M. Tomasulo, U.S. Pat. 7,790,068 B2: September 7, 2010
"Mechanism to Signal Protein–Ligand Interactions with Luminescent Quantum Dots": F. M. Raymo, M. Tomasulo, I. Yildiz, U.S. Pat. 8,198,099: June 12, 2012
"Colorimetric Detection of Cyanide with a Chromogenic Oxazine": F. M. Raymo, M. Tomasulo, U.S. Pat. 8,198,436: June 12, 2012
"Photochromic Compounds Based on Ring Opening and Closing of a [1,3]Oxazine Compound": F. M. Raymo, M. Tomasulo, U.S. Pat. 8,252,209: August 28, 2012
"Use of Oxazine Compounds for Making Chromogenic Materials": F. M. Raymo, M. Tomasulo, U.S. Pat. 8,304,537: November 12, 2012
"Photochemical Barcodes": Y. Zhang, S. Tang, F. M. Raymo, U.S. 62/645,574: March 20, 2018
Highlighted Publications
"A Photoactivatable Far-Red/Near-Infrared BODIPY to Monitor Cellular Dynamics in Vivo": L. Sansalone, S. Tang, J. Garcia-Amorós, Y. Zhang, S. Nonell, J. D. Baker, B. Captain and F. M. Raymo, ACS Sens., 2018, 3, 1347–1353
"Photochemical Barcodes": S. Tang, Y. Zhang, P. Dhakal, L. Ravelo, C. L. Anderson, K. M. Collins, F. M. Raymo, J. Am. Chem. Soc., 2018, 140, 4485−4488
"Highlighting Cancer Cells with Halochromic Switches": S. Tang, Y. Zhang, E. R. Thapaliya, A. S. Brown, J. N. Wilson, F. M. Raymo, ACS Sens., 2017, 2, 92–101
"A Photoswitchable Fluorophore for the Real-Time Monitoring of Dynamic Events in Living Organisms": Y. Zhang, S. Tang, L. Sansalone, J. D. Baker, F. M. Raymo, Chem. Eur. J., 2016, 22, 15027–15034
"A Photochromic Bioconjugate with Photoactivatable Fluorescence for Superresolution Imaging": J. Cusido, S. Shaban Ragab, E. R. Thapaliya, S. Swaminathan, J. Garcia-Amorós, M. J. Roberti, B. Araoz, M. M. A. Mazza, S. Yamazaki, A. M. Scott, F. M. Raymo, M. L. Bossi, J. Phys. Chem. C, 2016, 120, 12860–12870
ResearcherID Publications
Year Title Author(s) Source Volume